Methods for the isolation of Brucella abortus
نویسندگان
چکیده
منابع مشابه
The efficacy of bacteriological procedures for the isolation of Brucella abortus from abattoir material.
A process of emulsifying and centrifuging abattoir specimens before plating out is described. Brucella abortus was isolated more successfully by this process than by conventional methods, especially in low grade infections. The 5 different media used were equally effective in our attempts at isolation, but growth did not necessarily occur on all 5 plates. In dairy cows, specimens from supra-mam...
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Isolation and purification of major outer membrane proteins (OMP) from the cell wall envelope of Brucella abortus S-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (Tx-100) and lysozyme treatments, followed by ultracentrifugation. The crude OMP was treated with trypsin to free the preparation from any other protein contaminan...
متن کاملBrucella abortus endocarditis.
T HE purpose of this report is to describe the successful treatment of a case of bacterial endocarditis due to Brucella abortus. As far as I have been able to discover at the date of writing, no successfully treated case of this disease has been reported previously, with the exception of a case of mixed bacterial endocarditis presented in 1954 by Quinn and Brown.' A second case of treated Br. a...
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brucella transmission and epidemiology depend on infecting species and biovar. therefore, exact identification of the brucella is important to design correct control and treatment strategies. in this study, we examined presence of other brucellae in isfahan. one hundred twenty brucella isolates were collected and genomic dna was extracted from them. omp2a fragment of all isolates were amplified...
متن کاملRegulation of Brucella abortus catalase.
All aerobic organisms have mechanisms that protect against oxidative compounds. Catalase, peroxidase, superoxide dismutase, glutathione, and thioredoxin are widely distributed in many taxa and constitute elements of a nearly ubiquitous antioxidant metabolic strategy. Interestingly, the regulatory mechanisms that control these elements are rather different depending on the nature of the oxidativ...
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ژورنال
عنوان ژورنال: Hilgardia
سال: 1932
ISSN: 0073-2230
DOI: 10.3733/hilg.v06n12p355